|
KMID : 0379119840120010021
|
|
Korean Journal of Mycology
1984 Volume.12 No. 1 p.21 ~ p.26
|
|
|
Application of Glucose Oxidase for the Rapid Screening of Glucoamylase - producing Fungus
|
|
|
|
|
Abstract
|
|
|
|
Aspergillus awamori which produces glucoamylase was cultivated in the starch-Czapek-Dox´s medium in which sucrose was depleted. A rapid method for identification and assay of glucoamylase produced by the A awamori in the culture was established by the use of the glucose oxidase. The levels of glucose derived from the breakdown of the starch medium were assayed by using glucose oxidase, which was proved to be effective in the screening of glucoamylase-producing fungi in terms of rapid and pimple determination. After the cellulose acetate electrophoresis of the precipita ted culture broth, the glucoamylase hand in the gel was contacted with 2% starch solution with glucose oxidase, and then color reaction was occurred. Also this method could be effective to identify rapidly the fungal glucoamylase.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|